# Ozone and O3UV therapy > Medical ozone combined with ultraviolet light in a closed blood circuit. What the session involves, how long it takes, and who it is intended for. our signature therapy Medical Ozone + Ultraviolet Light Idledale, Colorado Hygeia's signature O3UV therapy combines medical ozone with ultraviolet light in one advanced blood-based therapy. Led by Holly Deffert, RN, each treatment is personalized to your individual wellness goals, with options ranging from foundational ozone support to high-dose O3UV protocols. Advanced therapy. Personalized care. A deeper approach to cellular wellness. [Book a 15-Minute Clarity Call→](https://app.malla.co/join/holly-deffert-10501/quick-clarity-call-15-minutes) [See O3UV Pricing→](https://ozoneandalternativetherapies.com/treatments/ozone-o3uv/#pricing) ## Who O3UV Therapy is for O3UV is for people who want to be proactive about their health, supporting overall wellness, vitality, recovery, and healthy aging, as well as those navigating more complex or chronic health concerns. For clients with chronic or more complex health concerns, Holly recommends starting with a [90-Minute Deep Dive Consultation ($350)](https://ozoneandalternativetherapies.com/treatments/personalized-wellness/#ways) and/or [KUREwave BioAnalysis](https://ozoneandalternativetherapies.com/kurewave-bioanalysis/). This allows Holly to look at the bigger picture, understand your health history and goals, and develop a more personalized roadmap before determining which therapies may be appropriate for your plan. Ozone therapy is not FDA-approved to diagnose, prevent, treat, or cure any disease, and results vary from person to person. in the chair One of our lovely patients receiving *O3UV*, already on her way to feeling so much better. Book a Free 15-Minute Clarity Call Not sure if ozone is right for you? Holly will listen to your history and tell you honestly whether O3UV fits your goals. [Book a 15-Minute Clarity Call→](https://app.malla.co/join/holly-deffert-10501/quick-clarity-call-15-minutes) ## How O3UV Therapy works O3UV combines medical ozone with ultraviolet blood irradiation (UVBI) in one blood-based therapy. During treatment, a measured amount of blood is drawn into a closed system, where it is exposed to a carefully controlled concentration of medical ozone and specific wavelengths of ultraviolet light before being returned to your circulation. ### Why combine ozone + UV light? Medical ozone and ultraviolet blood irradiation have each been studied independently for their effects on oxidative signaling, cellular processes, and immune response. O3UV brings these two modalities together in a single treatment. At Hygeia, your protocol is individualized based on your health history, goals, and overall wellness plan. Think of O3UV as cellular support, not a cure or a one-size-fits-all treatment. ## Our O3UV Therapies Hygeia offers two primary O3UV treatments. Each is administered by Holly Deffert, RN, with transparent pricing and clear time expectations. signature · most recommended ### O3UV Hygeia's foundational O3UV treatment combines medical ozone with ultraviolet blood irradiation. Approximately 60–120 mL of blood is treated during the procedure before being returned to circulation. Treatment time: approximately 45–60 minutes. Single treatments are available for clients who want to experience O3UV before committing to a series. Packages of 5 and 10 treatments are also available. $350 Optional Minor Autohemotherapy add-on: +$50 high dose ### High-Dose O3UV High-Dose O3UV combines ultraviolet blood irradiation with a significantly higher-volume ozone protocol. Approximately 600 mL of blood is treated, with a total ozone dose of up to 140,000 mcg depending on the individualized protocol. Treatment time: approximately 1.5–2 hours. Single treatments and packages of 5 and 10 treatments are available. $750 Optional Minor Autohemotherapy add-on: +$50 additional support ### Additional Ozone Support Depending on your individual needs and wellness plan, additional ozone applications may be incorporated when appropriate. These may include Minor Autohemotherapy, at-home ozone support, or Prolozone®/ozone joint therapy. Minor Autohemotherapy: $50 Add-On Minor Autohemotherapy is an optional add-on to O3UV or High-Dose O3UV. A small amount of the client's blood is ozonated and administered as an intramuscular (IM) injection. It may be incorporated into an individualized ozone protocol as additional wellness/immune support when appropriate. At-Home Ozone Support For clients interested in incorporating ozone into their home wellness routine, Holly can provide education and help them select appropriate home ozone equipment. Prolozone® / Ozone Joint Therapy Details for this therapy will be shared soon. Find the Right Ozone Plan Not sure whether O3UV or High-Dose O3UV is right for you? Holly can help you determine an appropriate starting point based on your health history, goals, and overall wellness plan. [Book a 15-Minute Clarity Call→](https://app.malla.co/join/holly-deffert-10501/quick-clarity-call-15-minutes) ## What to expect at your O3UV appointment Your O3UV experience begins with a brief check-in so we can review how you're feeling and determine the appropriate protocol for you. During treatment, blood is drawn into a closed system, exposed to a precise concentration of medical ozone and ultraviolet light, and then returned to your circulation. Depending on your individualized protocol, treatment volume and appointment length may vary. O3UV: approximately 45–60 minutes. High-Dose O3UV: approximately 1.5–2 hours. Treatment times are approximate and may vary. Most clients simply relax, read, work, or rest during their session. ### How many sessions will I need? The number of O3UV sessions varies depending on your goals, health history, and individual response. A single session is available for clients who want to experience O3UV before committing to a series. For clients pursuing a more comprehensive protocol, Hygeia offers packages of 5 and 10 treatments. Clients may begin with a 5-treatment package and add an additional five treatments when appropriate. Frequency and protocol can be adjusted along the way based on your individual response and overall wellness plan. ### Commonly used for Concerns clients have sought ozone and O3UV therapy to support. Listing them is not a claim that it treats, prevents, or cures any condition. ## Why clients choose Hygeia for O3UV At Hygeia, your care is personal from beginning to end. Holly Deffert, RN, personally performs your O3UV treatments, giving you consistency with someone who knows your history, your goals, and how you've responded along the way. With 16 years of nursing experience, including 14 years in emergency medicine, and a passion for integrative and regenerative wellness, Holly brings clinical experience together with a deeply individualized approach to care. O3UV can stand alone or become part of a bigger wellness strategy incorporating advanced testing, IV therapies, nutrition, detoxification support, and regenerative medicine when appropriate. Located in the Denver foothills, Hygeia serves Idledale, Evergreen, Morrison, Golden, Conifer, Genesee, and surrounding communities. No assembly-line wellness. No one-size-fits-all protocols. Just thoughtful, personalized care and a plan built around you. kind words ## What ozone *clients* say ★★★★★ ★★★★★ 14 weeks ago Nurse Holly of Hygeia delivered expert care for the healing of my severe cellulitis infection of my leg. I received both ozone and vitamin C infusions that greatly helped speed and complete my healing & recovery. Thank you so much! Fred Karres ★★★★★ ★★★★★ 6 weeks ago I came in for O3UV after months of feeling wiped out and getting nowhere with my regular doctor. Holly explained every step, never oversold anything, and by the third session I was sleeping through the night again. Danielle R. ★★★★★ ★★★★★ 3 months ago I do a maintenance O3UV every few weeks now. It is the one thing I have kept up for a year, because I can feel the difference when I skip it. Holly tracks everything and adjusts as we go. Mark T. packages ## Prepaid Sets and Protocol *Packages* O3UV: $350 per treatment High-Dose O3UV: $750 per treatment Packages of 5 and 10 available [See All Packages→](https://ozoneandalternativetherapies.com/pricing/) ## Frequently asked questions about ozone & O3UV Therapy ### 01 What is ozone therapy? Ozone therapy uses medical-grade ozone, a highly active form of oxygen, in precise doses. At Hygeia it is delivered through O3UV, where blood is ozonated, exposed to ultraviolet light and returned to circulation. It is used by integrative practitioners worldwide. Ozone therapy is not FDA-approved to diagnose, prevent, treat, or cure any disease, and O3UV is offered as wellness support rather than a treatment for disease. ### 02 What is the difference between O3UV and High-Dose O3UV? Both treatments combine medical ozone with ultraviolet blood irradiation. The primary differences are the amount of blood treated and the total amount of ozone used. Standard O3UV typically treats approximately 60–120 mL of blood using a lower total ozone dose and takes approximately 45–60 minutes. High-Dose O3UV treats approximately 600 mL of blood and uses a significantly higher total ozone dose, up to 140,000 mcg depending on the individualized protocol. High-Dose O3UV generally takes approximately 1.5–2 hours. The appropriate protocol is individualized based on the client and treatment plan. ### 03 How many O3UV treatments will I need? There is no one-size-fits-all number. Some clients begin with a single treatment, while others choose a series. Hygeia offers packages of 5 and 10 treatments, and frequency and protocol can be individualized based on your goals, health history, and response. ### 04 Is ozone therapy safe, and are there side effects? When administered correctly with medical-grade equipment and precise dosing, ozone therapy is generally well tolerated, which is why Holly, an RN, performs every treatment personally. As with any therapy, some people experience temporary effects. O3UV is not appropriate for everyone. Before treatment, Holly reviews your health history and relevant safety considerations to determine whether O3UV is appropriate for you. ### 05 Is ozone therapy FDA-approved? Ozone therapy is not approved by the FDA to diagnose, prevent, treat, or cure any disease, and we state that plainly. It is used by integrative and holistic practitioners around the world to support general wellness, and research is ongoing. Hygeia offers it as an alternative wellness option, with honest expectations and individualized guidance rather than promises of specific medical outcomes. ### 06 How much does ozone therapy cost at Hygeia? Standard O3UV is $350 per treatment and High-Dose O3UV is $750 per treatment. Packages of 5 and 10 treatments are available for clients completing a series. Package pricing can be viewed on our [Packages page](https://ozoneandalternativetherapies.com/pricing/). Minor Autohemotherapy may be added to an O3UV treatment for $50 when appropriate. ### 07 Can O3UV be incorporated into an ongoing wellness plan? Some clients choose O3UV as a short series, while others incorporate it into a longer-term wellness plan. Frequency and treatment intensity are individualized based on health history, goals, response, and the overall wellness plan. ## Holly Deffert, RN Holly Deffert is a registered nurse with 16 years of nursing experience, including 14 years in emergency medicine, and the founder of Hygeia Wellness. Her practice focuses on medical ozone and O3UV therapy, regenerative medicine and stem cell therapies, NAD+, advanced IV therapies, cellular health, and longevity. Holly personally works with each client, combining her clinical background with a research-informed, root-cause approach to create individualized wellness strategies. She also collaborates with naturopathic, functional-medicine, and holistic providers when appropriate to support the bigger picture of each client's health. Personalized care. Advanced wellness. A nurse who takes the time to look deeper. [Book your appointment→](https://ozoneandalternativetherapies.com/treatments/ozone-o3uv/#start) ## Ready to feel more like *yourself* again? O3UV combines medical ozone and ultraviolet light with nurse-led care built around you. Whether you want more vitality, better recovery, or a proactive approach to longevity, Holly will tell you honestly whether it belongs in your plan. Your health is an investment, and your wellness is a choice. Start with a 15-Minute Clarity Call or a 90-Minute Deep Dive, no pressure. [Book a 15-Minute Clarity Call→](https://app.malla.co/join/holly-deffert-10501/quick-clarity-call-15-minutes) [Book a 90-Minute Deep Dive: $350→](https://ozoneandalternativetherapies.com/treatments/personalized-wellness/#ways)